Flow-based Methods in Chemical Peptide and Protein Synthesis

Flow chemistry has emerged as a powerful method for on-demand chemical synthesis and modification of peptides proteins. Herein, we discuss the characteristics flow how they are applied to various aspects peptide chemistry. We highlight recent advances in automated flow-based synthesis, which extend length routinely accessible single-domain proteins allow collection time-resolved data. Applications this data prediction outcome potential development more sustainable methods also discussed. Finally, will review solutionphase approaches, including ligation strategies cyclization. Throughout review, current challenges future developments highlighted.